The aim of this study was to characterize the sarcoplasmic?endoplasmic reticulum Ca?ATPase (SERCA) isoforms in rabbit masticatory muscles compared with those in fast?twitch muscle. It was hypothesized that combined expression of the SERCA isoforms in fast? and slow?twitch muscles accounts for lower Ca?ATPase activity. SERCA was isolated by differential centrifugation, the isoforms were determined by ELISA, and the activity of each isoform was measured using a colorimetric method. Activity was tested for significance by anova, and the distribution of isoforms was assessed using the chi?square test (P < 0.05) and correlated to SERCA activity using Spearman’s rank correlation. SERCA1 was predominant (90.5%) in fast?twitch muscle, whereas a mixture of SERCA isoforms was fo…
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