RhoA plays a fundamental role in regulation of the actin cytoskeleton, intercellular attachment, and cell proliferation. During amelogenesis, ameloblasts (which produce the enamel proteins) undergo dramatic cytoskeletal changes and the RhoA protein level is up?regulated. Transgenic mice were generated that express a dominant?negative RhoA transgene in ameloblasts using amelogenin gene?regulatory sequences. Transgenic and wild?type (WT) molar tooth germs were incubated with sodium fluoride (NaF) or sodium chloride (NaCl) in organ culture. Filamentous actin (F?actin) stained with phalloidin was elevated significantly in WT ameloblasts treated with NaF compared with WT ameloblasts treated with NaCl or with transgenic ameloblasts treated with NaF, thereby confirming a block in the Rh…
MedWorm Sponsor Message: The blue sky scrub collection offers something for every medical professional. Offering some of the finest scrub clothing in the world.
